Indeed. Anything less would be unscientific.
Here are the tests I plan to run:
Weak Woolite solution soak
Strong Woolite solution soak
Woolite with enzyme stain remover pre-treat to waistband
Water with enzyme stain remover pre-treat to waistband
Woolite with detergent stain remover pre-treat to waistband
Water with detergent stain remover pre-treat to waistband
Woolite with Lanolin hand cleaner pre-treat to waistband
Water with lanolin hand cleaner pre-treat to waistband
I will not run the Oxy because the Great Zegna/Zanella/Red Knit Silk BB Tie Debacle of 2012 proved that the Oxy method is not color safe on wools and silks, especially red-based. (Note: I had no issue with the beige woolens I ran in that batch.) Since our goal is a system that removes the urine without ruining the trou, and we want it to be of general applicability, Oxy is out.
If you're counting along at home, this means I need no fewer than 9 pairs of wool trou, roughly equally soiled, that I'm willing to ruin (read: that cost me $1 or less). That could take me a while to amass, given that most GWs in my area charge on average $10 for all wool trou.