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Random health and exercise thoughts - Page 3765

post #56461 of 57266
Quote:
Originally Posted by Cool The Kid View Post

I do too but I do the bulk of my own cooking. Wifey was a vegetarian when we met, she still doesn't know jack shit about meat except that it's delicious
The only thing I am clearly better at cooking is steak, my fiancé makes the juiciest chicken I have had outside of a good restaurant.
post #56462 of 57266
Quote:
Originally Posted by venividivicibj View Post

Sorry, do you guys have a laymans explanation?

Beta oxidation just means you're burning fat as an energy source.

You do that when your blood sugar is low I.e between meals.
post #56463 of 57266
Quote:
Originally Posted by MGoCrimson View Post

Mice fed an isocaloric high fat, gluten inclusive diet gained more weight, more fat, had worse insulin sensitivity, higher baseline blood glucose and insulin, and lower amounts of enzymes involved in fat burning. 

I can't download the article on my phone but I am extremely wary of these findings and as you mention, it's one paper on one inbred animal model on one very specific diet in which gluten is actually a very small percentage of calories.

If you could provide some of the rest of the information about the study I'd be grateful. E.g.
- to what degree these findings were (50% less? How much weight gained? Etc etc)
- whether the macronutrient (not just calories) profiles were the same
- in what form the gluten was administered (cooked? Bread? With fibre?)
- how many mice

With the black 6 mice, it's a big advantage to use them as they're inbred so pretty much identical, which is great for getting statistically significant results, but does tend to throw things out in terms of proportions and actual applicability.
post #56464 of 57266
Quote:
Originally Posted by hendrix View Post


I can't download the article on my phone but I am extremely wary of these findings and as you mention, it's one paper on one inbred animal model on one very specific diet in which gluten is actually a very small percentage of calories.

If you could provide some of the rest of the information about the study I'd be grateful. E.g.
- to what degree these findings were (50% less? How much weight gained? Etc etc)
- whether the macronutrient (not just calories) profiles were the same
- in what form the gluten was administered (cooked? Bread? With fibre?)
- how many mice

With the black 6 mice, it's a big advantage to use them as they're inbred so pretty much identical, which is great for getting statistically significant results, but does tend to throw things out in terms of proportions and actual applicability.

 

Yea, heterogeneity has advantages and disadvantages and the mouse model has limited application to humans ofc.

 

Also, though not discussed in the above paper and although mouse BAT is a poor analogue for human BAT, its impaired activity on gluten inclusive diet has significant impact on FFA flux and total body energy homeostasis as indicated in this follow-up investigation http://www.ncbi.nlm.nih.gov/pubmed/26443339

 

* = p  < .05

** = p < .001

 

Weight*: 10% less

Epididymal fat*: 4.25% of BW vs 2.75% of BW 

Lipid content of visceral AT*: 850mg/g tissue vs 810mg/g tissue

Area of visceral AT**: 4200 μm² vs 2700 μm² 

 

Leptin gene expression*: 3x more than gf control

Serum leptin*: 32ng/ml vs 9ng/ml <--- ? I think they messed this one up

Resistin gene expression: not statiscally significant

Serum resistin*: 29ng/ml vs 19 ng/ml

Adiponectin gene expression*: 4x less than gf control

Serum adiponectin*: 114ng/ml vs 124 ng/ml

 

GLUT-4 expression*: 1/2 of gf control

IR expression*: 1/2 of gf control

PPARg expression*: 3.5x less than gf control

PPARa expression*: 2x less than gf control

HSL expression*: 5x less than gf control

CPT-1 expression*: 1/2 of gf control

 

Isocaloric and equal macronutrient composition at 25%c 60%f 15%p. No difference in food intake or fecal excretion of nutrients. Gluten form was not specified. I imagine it was isolated wheat gluten. n=24/group

 

GF control had 5x less CLS indicating less adipocytes in hypoxia. As a result, GF control also had lower levels of all pro-inflammatory cytokines TNFa, IL6,10 and chemokines MCP1/CCL2 that contribute to inflammation/IR.

post #56465 of 57266
Interesting. Which method to measure gene expression?
post #56466 of 57266
Quote:
Originally Posted by hendrix View Post

Interesting. Which method to measure gene expression?

Real-time semiquantitative PCR was performed using the Power SYBR MasterMix (Applied Biosystems, CA, USA) and specific primers in an ABI 7900 HT fast real-time PCR System (Applied Biosystems, CA, USA). The results were expressed relative to β-actin expression (foldincreased) or the ΔΔCt method 

post #56467 of 57266
Pretty standard method. It's never particularly consistently but if they're getting consistent results it's reasonable.

What were they doing the rt-pcr on though? Cells harvested from the mice or just a cell line? Quite different things.
post #56468 of 57266
Quote:
Originally Posted by hendrix View Post

Pretty standard method. It's never particularly consistently but if they're getting consistent results it's reasonable.

What were they doing the rt-pcr on though? Cells harvested from the mice or just a cell line? Quite different things.
Harvested from the respective groups
post #56469 of 57266
Ok. Well sounds legit then.
post #56470 of 57266
I love when this thread gets all nerdy.

It'll be interesting to see if the results are reproducible.
post #56471 of 57266

Hadn't seen my mom in a while.

 

Mom: Your arms are getting really big

Me: Really? I ...

Mom: It doesn't look good

 

:(

post #56472 of 57266
Critique my form please

https://www.dropbox.com/s/xazbsrha2bghkmj/Video%204-14-16%2C%205%2015%2002%20PM.mov?dl=0

I still feel a twinge in my back. Should I just switch to conventional?
post #56473 of 57266

@indesertum

 

Your technique and positions during the actual lift look pretty textbook to me.

 

The way you drop it looks a bit uncontrolled though. Its like a mix of a drop and a controlled negative.

 

I'd say either completely drop the bar from the top or really control it on the way down.

 

With your current negative phase it looks like you try to slow it down but eventually give out and round your back a bit.

post #56474 of 57266
Ahhh. That's a good point. Usually I kinda drop it first two reps and go for a controlled negative on the last rep. This was my last set and I think I gave up. Better to do slow negative all three times? I do 5x3 and then tons of accessory afterwards

But the slight hitch when I go up is ok? Does it just mean I have to start higher?

Also somebody told me paused squats are more for people who lift geared. Same with paused bench?
post #56475 of 57266
Controlled negatives and paused squats are good for everybody. They reinforce good motor patterns and build confidence in the hole
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